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tnf α catch antibody  (Miltenyi Biotec)


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    Miltenyi Biotec tnf α catch antibody
    Tnf α Catch Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tnf+secretion+assay/TNF-%CE%B1+Secretion+Assay+-+Detection+Kit+(APC)%2C+human/bio_rxiv__64898__2026__05__05__723030-167-12-16
    Average 97 stars, based on 7 article reviews
    tnf α catch antibody - by Bioz Stars, 2026-10
    97/100 stars

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    Incubation:

    Article Title: Salt-inducible kinase 3 protects tumor cells from cytotoxic T-cell attack by promoting TNF-induced NF-κB activation
    Article Snippet: .. After 12h incubation, T cells were collected and stained with the TNF secretion assay (catch assay) kit (Miltenyi biotech, Bergisch Gladbach, Germany) according to manufacturer ́s instructions. ..

    Staining:

    Article Title: Salt-inducible kinase 3 protects tumor cells from cytotoxic T-cell attack by promoting TNF-induced NF-κB activation
    Article Snippet: .. After 12h incubation, T cells were collected and stained with the TNF secretion assay (catch assay) kit (Miltenyi biotech, Bergisch Gladbach, Germany) according to manufacturer ́s instructions. ..



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    Image Search Results


    Specific IFN-γ and TNF-α release of T lymphocytes transduced with TIM-3-silenced HER2-specific chimeric antigen receptor (CAR) or HER2-specific CAR. (A, B) TIM-3-silenced CAR-T cells and control T cells were co-incubated with Galectin-9 + or Galectin-9 – SKOV3 tumor cells (E:T ratio 5:1 or 10:1). At 20 h after coculture, a specific enzyme-linked immunosorbent assay was used to analyze the supernatant for IFN-γ cytokine-release. Results were presented as mean ± standard deviation. (C, D) The detection of TNF-α in the same culture supernatant. Results were presented as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Journal: Genes & Diseases

    Article Title: Blockade of co-inhibitory receptor immune checkpoint protein TIM3/CD366 augments the anti-cancer activity of CAR-T therapy in solid tumors: An ovarian cancer example

    doi: 10.1016/j.gendis.2025.101978

    Figure Lengend Snippet: Specific IFN-γ and TNF-α release of T lymphocytes transduced with TIM-3-silenced HER2-specific chimeric antigen receptor (CAR) or HER2-specific CAR. (A, B) TIM-3-silenced CAR-T cells and control T cells were co-incubated with Galectin-9 + or Galectin-9 – SKOV3 tumor cells (E:T ratio 5:1 or 10:1). At 20 h after coculture, a specific enzyme-linked immunosorbent assay was used to analyze the supernatant for IFN-γ cytokine-release. Results were presented as mean ± standard deviation. (C, D) The detection of TNF-α in the same culture supernatant. Results were presented as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) was used to detect IFN-γ and tumor necrosis factor alpha (TNF-α) secretion (eBioscience) in accordance with the manufacturer's instructions.

    Techniques: Transduction, Control, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation